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Updated: May 6, 2026

High-throughput Physical Mapping of Chromosomes using Automated in situ Hybridization
Published on: June 28, 2012
Cytologically based physical maps of the group-2 chromosomes of wheat
D E Delaney1, S Nasuda, T R Endo
1Department of Plant Pathology, Kansas State University, 66506, Manhattan, Kansas, USA.
Abstract:
We have constructed cytologically based physical maps (CBPMs), depicting the chromosomal distribution of RFLP markers, of the group-2 chromosomes of common wheat (Triticum aestivum L. em Thell). Twenty-one homozygous deletion lines for 2A, 2B, and 2D were used to allocate RFLP loci to 19 deletion-interval regions. A consensus CBPM was colinearily aligned with a consensus genetic map of group-2 chromosomes. The comparison revealed greater frequency of recombination in the distal regions. Several molecularly tagged chromosome regions were identified which may be within the resolving power of pulsed-field gel electrophoresis. The CBPMs show that the available probes completely mark the group-2 chromosomes, and landmark loci for sub-arm regions were identified for targeted-mapping.
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