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Updated: May 6, 2026
Chromatin Immunoprecipitation ChIP to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
Published on: July 29, 2010
Stress-induced changes in gene interactions in human cells.
Renuka R Nayak1, William E Bernal, Jessica W Lee
1Medical Scientist Training Program, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA, Division of Rheumatology, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA, HHMI Medical Research Fellows Program, University of Pennsylvania, Philadelphia, PA 19104, USA, Department of Computer and Information Science, University of Pennsylvania, Philadelphia, PA 19104, USA, Department of Pediatrics, University of Pennsylvania, Philadelphia, PA 19104, USA, Department of Genetics, University of Pennsylvania, Philadelphia, PA 19104, USA and Howard Hughes Medical Institute, Chevy Chase, MD 20815, USA.
Human cells adapt to stress by altering gene interactions. A small set of genes gain new partners during stress, linking them to diseases like those involving endoplasmic reticulum stress or radiation sensitivity.
Area of Science:
- Cellular Biology
- Genomics
- Systems Biology
Background:
- Cells dynamically alter gene expression and interactions in response to environmental changes.
- Understanding cellular responses to stress is crucial for deciphering disease mechanisms.
Purpose of the Study:
- To investigate how human B cells modify gene expression and interactions under endoplasmic reticulum stress and ionizing radiation.
- To identify genes that change interaction partners during stress and their potential role in disease.
Main Methods:
- Analysis of >5000 gene expression levels in cultured human B cells from nearly 100 individuals.
- Construction of coexpression networks and inference of gene interactions.
- Application of machine learning to identify stress-specific gene interaction alterations.
Main Results:
- Thousands of genes were found to be induced or repressed by stress.
- Most gene interactions remained stable across baseline and stress conditions.
- A subset of genes acquired new interaction partners, forming stress-specific networks.
Conclusions:
- Genes altering their interaction partners during stress are linked to diseases involving endoplasmic reticulum stress or radiation sensitivity.
- Identifying genes with dynamic interaction changes, not just expression changes, is vital for understanding disease pathways.
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