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Light microscopic histochemistry on plastic sections.

Progress in Histochemistry and Cytochemistry

|January 1, 1985

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Semithin plastic sections offer superior microscopic images but hinder histochemical analysis. This review details methods to restore reactivity in plastic-embedded tissues for improved histochemical studies.

Area of Science:

Histochemistry
Microscopy
Biotechnology

Background:

Semithin plastic sections provide superior resolution and artifact reduction compared to traditional methods.
Plastic embedding, especially in epoxy resins, significantly reduces the reactivity of biological samples for histochemical analysis.
Reactivity is hindered by poor reagent penetration and blockade of chemical groups by fixatives and plastics.

Purpose of the Study:

To review histochemical methods applicable to plastic-embedded material.
To address challenges in applying histochemistry to semithin plastic sections.
To enable broader application of histochemical techniques in plastic-embedded samples.

Main Methods:

Utilizing polar plastics like glycol methacrylate for direct histochemical reactions.

Related Experiment Videos

Employing nonpolar epoxy resins with enhanced ultrastructure preservation.

Implementing matrix removal (alkoxides), oxidative treatments, and proteolytic digestion for epoxy sections.

Main Results:

Successful demonstration of proteins, carbohydrates, lipids, nucleic acids, amines, ions, and enzymes in plastic sections.
Polar plastics allow direct histochemistry but offer less ideal ultrastructure.
Nonpolar plastics require pre-treatment to restore histochemical reactivity.

Conclusions:

Histochemical analysis of plastic-embedded tissues is feasible with adapted methods.
A narrower range of histochemical methods is available for plastic sections compared to paraffin or frozen sections.
Further research can expand the utility of histochemistry in plastic-embedded samples.