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Published on: June 16, 2020
Isozyme diversity in sour, sweet, and ground cherry
J A Beaver1, A F Iezzoni, C W Ramm
1Department of Horticulture, Michigan State University, 48824, East Lansing, MI, USA.
Isozyme analysis revealed distinct genetic divergence between diploid sweet cherries and tetraploid sour, ground, and hybrid cherries. Tetraploid cherry species exhibit significantly higher heterozygosity than diploid sweet cherries.
Area of Science:
- Plant genetics
- Biochemistry
- Horticulture
Background:
- Cherry species (Prunus cerasus L., P. avium L., P. fruticosa Pall.) exhibit diverse ploidy levels.
- Understanding genetic diversity and heterozygosity is crucial for cherry breeding and conservation.
Purpose of the Study:
- To investigate isozyme divergence among sour, sweet, and ground cherry species.
- To determine enzyme polymorphisms and heterozygosity levels between diploid and tetraploid cherries.
Main Methods:
- Evaluation of seven enzyme systems (PGI, IDH, PGM, 6-PGD, LAP, SKDH, MDH) in 36 cherry selections.
- Principal coordinate analysis (PCoA) to assess isozyme divergence.
- Analysis of enzyme polymorphisms and heterozygosity in an additional 86 selections.
Main Results:
- Principal coordinate analysis separated diploid sweet cherries from tetraploid sour, ground, and hybrid cherries.
- 6-phosphogluconate dehydrogenase (6-PGD) was the most polymorphic enzyme, showing 16 patterns.
- Tetraploid cherry species displayed higher average heterozygosity (78%) compared to diploid sweet cherries (19%).
Conclusions:
- Isozyme analysis effectively differentiates cherry species based on ploidy.
- Tetraploid cherry species possess greater genetic variability and heterozygosity than diploid sweet cherries.
- Findings provide insights into cherry genetic resources for breeding programs.
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