Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

RACE - Rapid Amplification of cDNA Ends

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Defining the temporal evolution of gut dysbiosis and inflammatory responses leading to hepatocellular carcinoma in Mdr2 -/- mouse model.

BMC microbiology·2021

Same author

RETRACTION: The roles of the cation transporters CHX21 and CHX23 in the development of Arabidopsis thaliana.

Journal of experimental botany·2021

Same author

Identification of genomic region(s) responsible for high iron and zinc content in rice.

Scientific reports·2019

Same author

Modulation of endochondral ossification by MEK inhibitors PD0325901 and AZD6244 (Selumetinib).

Bone·2013

Same author

Comparative genetic analyses of metric traits using diallel and factorial mating designs in bread wheat.

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·2013

Same author

Isolation of protoplasts and regeneration of callus from suspension cultures of cultivated beets.

Plant cell reports·2013

Search research articles

Related Experiment Video

Updated: May 6, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

The identification of duplicate accessions within a rice germplasm collection using RAPD analysis.

P S Virk¹, H J Newbury, M T Jackson

¹School of Biological Sciences, University of Birmingham, B15 2TT, Edgbaston, Birmingham, UK.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 1, 2013

Summary

Researchers used Random Amplified Polymorphic DNA (RAPD) analysis to identify duplicate rice accessions. This method accurately distinguishes between true and suspected duplicates in genetic resources collections.

More Related Videos

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Related Experiment Videos

Last Updated: May 6, 2026

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA

Published on: January 22, 2018

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Area of Science:

Agricultural Science
Genetics
Molecular Biology

Background:

Genetic resources collections are vital for crop improvement.
Accurate identification and management of germplasm are crucial for maintaining biodiversity.
Identifying duplicates is essential to prevent redundancy and optimize resource allocation.

Purpose of the Study:

To develop and validate a method for identifying duplicate accessions within the Oryza sativa germplasm collection.
To determine the effectiveness of Random Amplified Polymorphic DNA (RAPD) analysis for discriminating between true and suspected duplicates.
To propose generalizable procedures for duplicate identification in genetic resource collections.

Main Methods:

Random Amplified Polymorphic DNA (RAPD) analysis was performed on a set of Oryza sativa accessions.
The number of primers, polymorphic bands, and total bands were quantified.
Statistical analysis was employed to assess the discriminatory power of RAPD markers.

Main Results:

RAPD analysis successfully discriminated between known and suspected duplicate rice accessions.
Specific primer combinations and band profiles were identified for accurate identification.
The study established parameters for effective duplicate detection using RAPD markers.

Conclusions:

RAPD analysis is a reliable and efficient tool for identifying duplicate accessions in rice genetic resources.
The proposed procedures can be broadly applied to other genetic resource collections.
Effective duplicate management enhances the utility and conservation of valuable germplasm.