Molecular cloning and characterization of the chloroplast ribosomal RNA genes from Spirodela oligorhiza
Related Experiment Video
Updated: May 6, 2026

Analysis of Protein Import into Chloroplasts Isolated from Stressed Plants
Published on: November 1, 2016
R J Keus1, D J Roovers, H van Heerikhuizen
1Biochemical Laboratory, Free University, de Boelclaan 1083, 1081, HV Amsterdam, The Netherlands.
Abstract:
The organization of the chloroplast ribosomal RNA genes in Spirodela oligorhiza has been determined. We have therefore characterized two cloned BamHI fragments containing the genes for the large and small rRNA genes, and two PstI fragments, each containing one of the two large, invertedly repeated regions of chloroplast DNA. Characterization was performed by restriction endonuclease mapping, DNA/RNA hybridization and electronmicroscopic R-loop analysis.The results show that the rRNA genes are present in the order 16S, 23S, 4.5S and 5S. The 16S (1.5 kb) and 23S (2.9 kb) rRNA genes are separated by a spacer of 2.25 kb. There appears to be a close resemblance between the organization of the chloroplast rRNA genes in Spirodela oligorhiza and other higher plants so far examined.
Related Concept Videos
Protein Transport to the Inner Chloroplast Membrane
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Protein Transport to the Stroma
Protein complexes called the translocon of the outer chloroplast membrane or TOC complex, and the translocon of the inner chloroplast membrane or TIC complex mediate the...
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes
Protein Transport to the Outer Chloroplast Membrane
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.

