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Updated: May 6, 2026

Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
Published on: March 30, 2015
The need for transparency and good practices in the qPCR literature
Stephen A Bustin1, Vladimir Benes, Jeremy Garson
1Postgraduate Medical Institute, Anglia Ruskin University, Chelmsford, UK.
Abstract:
Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.
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