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Intra- and inter-specific variations in Lens revealed by RAPD markers
A Abo-Elwafa1, K Murai, T Shimada
1Research Institute of Agricultural Resources, Ishikawa Agricultural College, 1-308 Suematsu, Nonoichi-machi, 921, Ishikawa, Japan.
Summary
Randomly amplified polymorphic DNA (RAPD) markers reveal genetic diversity in the Lens genus. Wild lentil species show higher variation than cultivated types, with L. culinaris ssp. orientalis being a likely progenitor.
Area of Science:
- Plant genetics
- Molecular markers
- Crop wild relatives
Background:
- The genus Lens, encompassing cultivated lentil (Lens culinaris) and its wild relatives, is important for global food security.
- Understanding genetic diversity within Lens is crucial for crop improvement and conservation efforts.
- Randomly amplified polymorphic DNA (RAPD) markers offer a cost-effective method for assessing genetic variation.
Purpose of the Study:
- To estimate intra- and interspecific genetic variations within the genus Lens using RAPD markers.
- To evaluate genetic diversity among cultivated lentil (L. culinaris ssp. culinaris) cultivars and wild Lens species.
- To identify potential progenitor species for cultivated lentil and assess relationships between microsperma and macrosperma types.
Main Methods:
- Utilized 40 random 10-mer primers to analyze genetic variability in 20 L. culinaris ssp. culinaris cultivars and 16 wild Lens accessions.
- Scored 50 reproducible DNA bands from ten primers, with 90% exhibiting polymorphism.
- Calculated genetic distances using simple matching coefficients and constructed a dendrogram via UPGMA to visualize genetic relationships.
Main Results:
- Intraspecific variation in cultivated lentil was generally lower than in wild Lens species, with the exception of L. ervoides.
- Lens culinaris ssp. orientalis emerged as the most probable progenitor of cultivated lentil.
- Microsperma and macrosperma lentil cultivars were genetically indistinguishable using the employed RAPD markers.
Conclusions:
- Wild Lens species harbor greater genetic diversity than cultivated lentils, highlighting their importance for breeding programs.
- L. culinaris ssp. orientalis is a strong candidate for the ancestral origin of cultivated lentil.
- RAPD markers, in this study, could not differentiate between small-seeded (microsperma) and large-seeded (macrosperma) lentil cultivars, suggesting limited marker utility for this distinction.
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