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Mapping the Sw-5 locus for tomato spotted wilt virus resistance in tomatoes using RAPD and RFLP analyses.

M R Stevens1, E M Lamb, D D Rhoads

  • 1Department of Horticulture and Forestry, University of Arkansas, 72701, Fayetteville, AR, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|November 1, 2013
PubMed
Summary

Researchers mapped the Sw-5 gene, which provides resistance to tomato spotted wilt virus (TSWV). This gene was located on chromosome 9 using molecular markers, aiding in breeding resistant tomato varieties.

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Area of Science:

  • Plant genetics
  • Molecular biology
  • Crop science

Background:

  • The Sw-5 locus in tomato (Lycopersicon esculentum) confers dominant resistance to tomato spotted wilt virus (TSWV).
  • Identifying molecular markers linked to Sw-5 is crucial for marker-assisted selection in breeding programs.
  • Developing near-isogenic lines (NILs) and interspecific populations aids in gene mapping.

Purpose of the Study:

  • To map the genetic location of the Sw-5 resistance locus.
  • To identify molecular markers linked to the Sw-5 locus for breeding applications.
  • To facilitate the development of TSWV-resistant tomato cultivars.

Main Methods:

  • Development of near-isogenic lines (NILs) and an F2 interspecific population (L. esculentum x L. pennellii).
  • Random Amplified Polymorphic DNA (RAPD) analysis using 748 random 10-mer oligonucleotides on NILs.
  • Co-segregation analysis of resistance phenotype with Restriction Fragment Length Polymorphisms (RFLPs) in the F2 population.

Main Results:

  • A specific RAPD marker (primer GAGCACGGGA, ~2200 bp band) showed linkage to the Sw-5 locus.
  • The Sw-5 locus was physically mapped to chromosome 9, positioned between markers CT71 and CT220.
  • This region is located near the telomere of the long arm of chromosome 9.

Conclusions:

  • The study successfully identified a RAPD marker linked to the Sw-5 resistance gene.
  • The precise mapping of Sw-5 provides valuable information for breeding TSWV-resistant tomatoes.
  • These findings contribute to the genetic understanding and improvement of tomato disease resistance.