Bacillus enclensis sp. nov., isolated from sediment sample.

Area of Science:

• Microbiology
• Bacterial Taxonomy
• Genomics

Background:

• Isolation of novel microorganisms is crucial for understanding microbial diversity and potential applications.
• The genus Bacillus is known for its diverse metabolic capabilities and ecological significance.

Purpose of the Study:

• To characterize a novel bacterial isolate, strain SGD-1123(T).
• To determine the taxonomic position of strain SGD-1123(T) within the genus Bacillus.
• To propose a new species name for the isolate.

Main Methods:

• Phenotypic characterization including growth conditions (temperature, pH, salinity).
• Chemotaxonomic analysis of whole-cell hydrolysates, fatty acids, menaquinone, and polar lipids.
• Phylogenetic analysis based on 16S rRNA gene sequencing and genomic DNA G+C content determination.

Main Results:

• Strain SGD-1123(T) exhibits tolerance to a wide range of temperatures (15-42 °C), pH (5-12), and NaCl concentrations (0-12%).
• Chemotaxonomic data revealed meso-diaminopimelic acid, galactose, arabinose, iso-C15:0, anteiso-C15:0 fatty acids, MK-7 menaquinone, and specific polar lipids.
• Phylogenetic analysis placed the strain within the Bacillus genus, closely related to Bacillus aquimaris, Bacillus vietnamensis, and Bacillus marisflavi, with 94.5% 16S rRNA gene sequence similarity to B. aquimaris.

Conclusions:

• Strain SGD-1123(T) represents a novel species of the genus Bacillus.
• The proposed name for this new species is Bacillus enclensis.
• The type strain is SGD-1123(T) (NCIM 5450(T)=CCTCC AB 2011125(T)).