Effect of methylprednisolone and edaravone administration on spinal cord injury

Spinal cord injury (SCI) is one of the most devastating traumatic conditions that primarily affects young males with an annual incidence of 15-40 cases per million.

To explore the superior neuroprotective effect of edaravone (ED) on spinal cord injury during maintenance therapy compared with methylprednisolone (MP).

Sprague-Dawley rat model of spinal cord injury was established by modified Allen's method. Total 114 rats were divided into two groups and then six subgroups individually: A1 (control group, normal saline injection within 8 h), B1 (MP group, MP injection within 8 h), C1(ED group, ED injection within 8 h), A2 (control group, normal saline injection after 8 h), B1 (MP group, MP injection after 8 h), C1 (ED group, ED injection after 8 h). Further, we investigated the changes of histopathology, caspase-3 and Bcl-xL positive cell.

Haemorrhage, swelling, hyperaemia, gliocytes hyperplasia, inflammatory cells infiltration, vacuolar denaturation, and nucleus concentration could be observed, especially in control group. Caspase-3 positive cell was significantly decreased in MP and ED group within 8 h administration, but caspase-3 positive cell was only significantly decreased in ED group after 8 h administration. And B-cell lymphoma extra large (Bcl-xL) was significantly increased in ED group than MP group no matter within 8 h or after 8 h administration.

More attention should be paid on the time point of MP administration, and ED administration seem to be more effective for maintenance therapy.