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Antioxidant and toxicological evaluation of Cassia sopherain streptozotocin-induced diabetic Wistar rats
Rambir Singh1, Priyanka Bhardwaj, Poonam Sharma
1Department of Biomedical Sciences, Bundelkhand University, Jhansi, India.
Background:
Multiple-organ failure is the main cause of death in diabetes mellitus (DM). Hyperglycemia-induced oxidative stress is responsible for major diabetic complications, including multiple-organ failure. Medicinal plants possessing antioxidant activity may reduce oxidative stress and improve the functions of various organs affected by hyperglycemia.
Objectives:
This study was designed to evaluate the antioxidant effect of Aqueous Extract of Cassia sophera (AECS) in streptozotocin (STZ)-induced diabetic Wistar rats.
Materials And Methods:
AECS (200 mg/kg body weight (bw)) and the standard antidiabetic drug glibenclamide (10 mg/kgbw) were administered orally by gavaging for 28 days.
Results:
Oral administration of AECS inhibited STZ-induced increase in lipid peroxidation (LPO), aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), bilirubin, creatinine and urea in liver of diabetic rats. Significant increase in activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx), and a reduced level of glutathione (GSH), were observed in the liver, kidney, pancreas and testis on AECS treatment.
Conclusion:
The results demonstrate that AECS is not only useful in controlling blood glucose, but also has antioxidant potential to protect the liver, kidney, pancreas and testis against damage caused by hyperglycemia-induced oxidative stress.
Insights
Aqueous Extract of Cassia sophera (AECS) demonstrates significant antioxidant potential, protecting organs from hyperglycemia-induced damage in diabetic rats. This natural extract helps control blood glucose and reduces oxidative stress markers.
Area of Science:
- Biochemistry
- Pharmacology
- Toxicology
Background:
- Diabetes mellitus (DM) complications, including multiple-organ failure, are primarily driven by hyperglycemia-induced oxidative stress.
- Medicinal plants with antioxidant properties offer a potential therapeutic strategy to mitigate diabetic complications.
- Cassia sophera is explored for its potential to counteract hyperglycemia-related organ damage.
Purpose of the Study:
- To evaluate the antioxidant efficacy of Aqueous Extract of Cassia sophera (AECS).
- To assess the protective effects of AECS against streptozotocin (STZ)-induced diabetes in Wistar rats.
Main Methods:
- Oral administration of AECS (200 mg/kg bw) and glibenclamide (10 mg/kg bw) to STZ-induced diabetic Wistar rats for 28 days.
- Biochemical analysis of liver enzymes (AST, ALT, ALP), bilirubin, creatinine, urea, and oxidative stress markers (LPO, SOD, CAT, GPx, GSH) in various organs.
Main Results:
- AECS administration significantly inhibited the STZ-induced increase in lipid peroxidation (LPO) and liver enzymes (AST, ALT, ALP), bilirubin, creatinine, and urea.
- AECS treatment led to a significant increase in antioxidant enzyme activities (SOD, CAT, GPx) and a decrease in glutathione (GSH) levels in the liver, kidney, pancreas, and testis.
- These findings indicate a reduction in hyperglycemia-induced oxidative stress and organ damage.
Conclusions:
- AECS exhibits potent antioxidant activity, effectively protecting the liver, kidney, pancreas, and testis from hyperglycemia-induced oxidative stress.
- The study confirms AECS's dual role in managing blood glucose levels and mitigating diabetic organ damage.
- AECS represents a promising natural therapeutic agent for managing diabetic complications associated with oxidative stress.
