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Absorption mode FTICR mass spectrometry imaging.

Donald F Smith1, David P A Kilgour, Marco Konijnenburg

  • 1FOM Institute AMOLF , Science Park 104, Amsterdam, North Holland, 1098 XG The Netherlands.

Analytical Chemistry
|November 2, 2013
PubMed
Summary

Absorption mode enhances Fourier transform ion cyclotron resonance mass spectrometry imaging by improving mass resolving power and image contrast. This technique reveals previously unresolved spatial and mass features for better molecular imaging.

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Area of Science:

  • Analytical Chemistry
  • Molecular Imaging
  • Spectrometry

Background:

  • Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) provides high mass resolving power crucial for molecular imaging.
  • Higher magnetic fields are typically used to enhance mass resolving power.
  • Phase correction offers an alternative method to increase mass resolving power.

Purpose of the Study:

  • To introduce and evaluate the use of absorption mode for FTICR-MS imaging.
  • To demonstrate the benefits of phase correction in FTICR-MS imaging data.

Main Methods:

  • Utilized the Autophaser algorithm for phase correction of spectral data.
  • Employed Chameleon workflow-based software to generate absorption mode "Datacubes" for analysis.
  • Applied FTICR-MS imaging to molecular imaging experiments.

Main Results:

  • Absorption mode processing significantly improved mass resolving power compared to conventional magnitude mode.
  • Enhanced mass accuracy and signal-to-noise ratio were observed in absorption mode.
  • New mass and spatial features, unresolvable in magnitude mode, were revealed, leading to improved image contrast.

Conclusions:

  • Absorption mode is effectively applied to FTICR-MS imaging for the first time.
  • Phase correction via the Autophaser algorithm and Chameleon software enhances FTICR-MS imaging capabilities.
  • Absorption mode FTICR-MS imaging offers superior resolution and contrast for molecular imaging applications.