Related Experiment Video

Updated: May 6, 2026

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment
11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

2.3K

Phosphatidylinositol 3-kinase (PI3KCA) oncogene mutation analysis and gene expression profiling in primary breast

Mahesh Kandula1, Kalyan Kumar Chennaboina, Ammi Raju Ys

  • 1Department of Biotechnology, Krisani Biosciences Private Limited, India

Asian Pacific Journal of Cancer Prevention : APJCP
|November 2, 2013
PubMed
Abstract

Insights

Mutations in the PIK3CA gene were found in 34% of breast cancers, with higher gene expression in tumor tissues. PIK3CA mutation detection may aid in breast cancer diagnosis and treatment selection.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The phosphatidylinositol 3-kinase (PI3K) pathway is crucial for cell apoptosis, proliferation, and motility.
  • Investigating the PIK3CA gene's role in breast carcinomas is essential for understanding cancer development.

Purpose of the Study:

  • To analyze mutations and gene expression profiles of the PIK3CA gene in breast carcinomas.
  • To determine the potential role of PIK3CA alterations in breast cancer.

Main Methods:

  • Direct DNA sequencing of PIK3CA hotspots (exons 9 and 20) in 38 breast cancer biopsy samples.
  • Quantitative analysis of PIK3CA gene expression at the mRNA level using RT-PCR in tumor and control tissues.
  • Statistical analysis using Fisher's exact test to correlate mRNA levels.

Main Results:

  • Somatic mutations in PIK3CA were identified in 13 out of 38 cases (34% of patients).
  • Specific mutations found include G>A (E545K) in exon 9 (9/13 cases) and A>G in exon 20 (4/13 cases).
  • Significantly higher PIK3CA mRNA expression was observed in breast cancer tissues compared to control tissues (p=0.001).

Conclusions:

  • PIK3CA mutations are prevalent in breast cancer and represent a potential emerging tumor marker.
  • Detection of PIK3CA mutations may hold significant clinical implications for breast cancer diagnosis, progression monitoring, and therapeutic strategy.
  • Future applications may include utilizing PIK3CA mutation status to guide treatment selection.

More Related Videos

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases07:41

Performing Data Mining And Integrative Analysis Of Biomarker in Breast Cancer Using Multiple Publicly Accessible Databases

Published on: May 17, 2019

8.6K
Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures08:02

Phospholipid Mediator Induced Transformation in Three-Dimensional Cultures

Published on: July 27, 2022

2.0K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
5.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.6K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
7.8K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.2K
Cancer-Critical Genes I: Proto-oncogenes01:33

Cancer-Critical Genes I: Proto-oncogenes

6.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
8.3K