Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A new method for studying epidermalization in vitro.

B Coulomb, P Saiag, E Bell

    The British Journal of Dermatology
    |January 1, 1986
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Regional anaesthesia in Video or Robot assisted thoracic surgery: A French multicentre prospective observational cohort study (ARTIST study).

    Anaesthesia, critical care & pain medicine·2026
    Same author

    The AADOCR Position Statement on Community Water Fluoridation.

    Journal of dental research·2025
    Same author

    Exposure-response relationship of niraparib in maintenance therapy for recurrent ovarian cancer: ancillary analysis of the French GINECO-NiQoLe study.

    ESMO open·2025
    Same author

    Lurbinectedin in extensive-stage small-cell lung cancer: a brief report of the IFCT-2105 LURBICLIN study.

    ESMO open·2024
    Same author

    Efficacy of subsequent therapies in patients with advanced ovarian cancer who relapse after first-line olaparib maintenance: results of the PAOLA-1/ENGOT-ov25 trial.

    Annals of oncology : official journal of the European Society for Medical Oncology·2024
    Same author

    Spectrofluorimetric determination of acetone in water samples with solid-phase extraction and a new benzaldehyde derivative.

    Talanta·2024
    Same journal

    Shallow Cuts, Deeper Issues: The Primacy of Deep Margins in Cutaneous Squamous Cell Carcinoma.

    The British journal of dermatology·2026
    Same journal

    Management Patterns and Repigmentation Outcomes in Pediatric Segmental Versus Non-segmental Vitiligo: A Single-center Retrospective Study (2015-2024).

    The British journal of dermatology·2026
    Same journal

    Quality-of-Life Measurement in Migrants and Other Vulnerable Groups: Experience with Skindex-16 in Malta and Lebanon.

    The British journal of dermatology·2026
    Same journal

    Does loss of chromosome Y in blood contribute to the sex disparity of dermatitis and eczema at older ages?

    The British journal of dermatology·2026
    Same journal

    Soluble CD83 reverses alopecia areata via IDO-mediated Treg cell activation.

    The British journal of dermatology·2026
    Same journal

    Durability of Response to Icotrokinra in Adults With Moderate-to-Severe Plaque Psoriasis: One-Year Results From the Phase 3, Placebo- and Active Comparator-Controlled ICONIC-ADVANCE 1 & ICONIC-ADVANCE 2 Trials.

    The British journal of dermatology·2026
    See all related articles

    Researchers developed a novel in vitro method for studying epidermalization using punch biopsies in a dermal equivalent. This technique yields a large quantity of differentiated epidermis, valuable for research and potential skin grafting applications.

    Area of Science:

    • Dermatology
    • Tissue Engineering
    • Cell Biology

    Background:

    • Studying epidermalization in vitro is crucial for understanding skin development and disease.
    • Existing methods like explant cultures and keratinocyte cultures have limitations.

    Purpose of the Study:

    • To describe a new, effective in vitro method for studying epidermalization.
    • To provide a quantitative system for epidermal research and potential therapeutic applications.

    Main Methods:

    • A punch biopsy was inserted into a collagen matrix containing fibroblasts (dermal equivalent).
    • Fibroblast contraction created a stable dermal equivalent.
    • A specific culture medium promoted epidermal growth from the biopsy.
    • Epidermal differentiation was assessed via histology and keratin expression.

    Related Experiment Videos

    Main Results:

    • A new epidermis formed around the biopsy, growing linearly over time.
    • The in vitro epidermis exhibited differentiation markers similar to in vivo epidermis.
    • Key markers included cuboidal basal cells, keratohyalin granules, and specific keratin subsets (65-67 kd).

    Conclusions:

    • The described method offers a robust in vitro model for epidermalization.
    • It combines advantages of explant and cell culture techniques.
    • The model is suitable for pharmacological studies and as a potential material for skin grafting.