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Olive leaf down-regulates the oxidative stress and immune dysregulation in streptozotocin-induced diabetic mice
Jung-Hyun Park1, Ji-Hye Jung, Jin-Young Yang
1Division of Biological Science, College of Science, Sookmyung Women's University, Seoul, Korea.
Abstract:
Type 1 diabetes is an endocrinologic disorder characterized by uncontrolled glucose regulation and oxidative stress. Olive leaves have been studied extensively for their antioxidant activity and capacity to improve immune function. We hypothesized that olive leaf powder supplementation will be effective in inhibiting the oxidative stress and immune dysregulation in streptozotocin (STZ)-induced diabetic mice. Mice were assigned to 1 of 5 groups: control (C), STZ-induced diabetes (D), and STZ-induced diabetes supplemented with very low dose (VLOL), low dose (LOL), or high dose of olive leaf powder (HOL). Blood glucose in the VLOL and LOL groups was lower than that in the D group (P < .05). Insulin levels were increased in all experimental groups in comparison with that in the D group, (P < .05). Superoxide dismutase, glutathione peroxidase, and catalase activities were shown to decrease in the D group, whereas these were increased in the VLOL and LOL groups. Nitric oxide levels decreased in the VLOL and LOL groups, as compared with the D group. The messenger RNA expression levels of inducible nitric oxide synthase were significantly decreased in the VLOL and HOL groups, and interferon-γ levels were significantly decreased in the liver of the VLOL, LOL, and HOL groups compared with the levels in the D group. Interleukin-17 levels were significantly decreased in the VLOL and HOL groups. Th1 and Th17 cytokine levels were increased in the D group but decreased in all the experimental groups. Th2 cytokine levels were increased in all olive leaf-supplemented groups compared with those in the D group. These results indicate a reduction in the levels of proinflammatory cytokines, suggesting that olive leaves have the potential to provide therapeutic inhibition of diabetic complications.
Insights
Olive leaf powder supplementation effectively reduced oxidative stress and immune dysregulation in type 1 diabetes models. This suggests olive leaves may offer therapeutic benefits for managing diabetic complications.
Area of Science:
- Endocrinology
- Immunology
- Nutritional Science
Background:
- Type 1 diabetes involves impaired glucose regulation and oxidative stress.
- Olive leaves possess antioxidant properties and immune-modulating potential.
Purpose of the Study:
- To investigate the efficacy of olive leaf powder in mitigating oxidative stress and immune dysregulation in streptozotocin-induced diabetic mice.
Main Methods:
- Mice were divided into control, diabetic, and diabetic groups supplemented with varying doses of olive leaf powder.
- Key biochemical markers, antioxidant enzyme activities, and cytokine levels were assessed.
Main Results:
- Olive leaf supplementation lowered blood glucose and increased insulin levels.
- Antioxidant enzyme activities (superoxide dismutase, glutathione peroxidase, catalase) were restored.
- Pro-inflammatory cytokine levels (IFN-γ, IL-17, Th1, Th17) decreased, while Th2 cytokines increased.
Conclusions:
- Olive leaf powder demonstrates potential therapeutic effects against diabetic complications.
- It may inhibit oxidative stress and modulate immune responses in type 1 diabetes.
