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An aptamer-based biosensor for sensitive thrombin detection with phthalocyanine@SiO2 mesoporous nanoparticles.

Zhou Jiang1, Tingting Yang, Minyan Liu

  • 1Institute of Research on Functional Materials, Cancer Metastasis Alert and Prevention Center, Fuzhou University, Fuzhou 350108, China; College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350108, China.

Biosensors & Bioelectronics
|November 2, 2013
PubMed
Summary

This study developed silica nanoparticles loaded with a photosensitizer to detect human thrombin. The novel aptamer-based biosensor offers high sensitivity for biomarker screening.

Keywords:
ChemiluminescenceSilica nanoparticleTetra-α-(24-di-tert-butylphenoxy)-phthalocyaninato zincThrombin

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Area of Science:

  • Nanotechnology and Materials Science
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Hydrophobic photosensitizers often suffer from poor aqueous solubility, limiting their application in biological systems.
  • Developing sensitive and selective biosensors for early disease detection is crucial for clinical diagnostics.

Purpose of the Study:

  • To synthesize silica nanoparticles encapsulating a hydrophobic photosensitizer (ZnPc(OAr)4) to improve its aqueous solubility and photophysical properties.
  • To develop a photoinduced chemiluminescence (CL) aptamer-based biosensor for the sensitive detection of human thrombin.

Main Methods:

  • Synthesis of monodispersed silica nanoparticles (approx. 100 nm) via co-hydrolysis of TEVS and APTES, encapsulating ZnPc(OAr)4.
  • Characterization of nanoparticle properties, including Q-band absorption and singlet oxygen generation.
  • Development of an aptamer-based sandwich assay utilizing photoinduced CL for thrombin detection.

Main Results:

  • Successfully synthesized silica nanoparticles with improved aqueous solubility and photoluminescence of the encapsulated ZnPc(OAr)4.
  • Demonstrated efficient singlet oxygen generation by the nanoparticles upon irradiation.
  • Achieved highly selective detection of human thrombin with a low detection limit of 80 pmol/L using the developed aptamer-based biosensor.

Conclusions:

  • Silica nanoparticle encapsulation is an effective strategy to enhance the performance of hydrophobic photosensitizers in aqueous environments.
  • The developed photoinduced CL aptamer-based biosensor demonstrates high sensitivity and selectivity for thrombin detection.
  • This approach is promising for screening biomarkers in complex biological samples at ultratrace levels.