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Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells
10:22

Isolation of Viral Replication Compartment-enriched Sub-nuclear Fractions from Adenovirus-infected Normal Human Cells

Published on: November 12, 2015

Adenovirus-induced leakage of co-endocytosed macromolecules into the cytosol

A Yoshimura

    Cell Structure and Function
    |December 1, 1985
    PubMed

    Insights

    Adenovirus enhances the uptake of macromolecules like fluorescein isothiocyanate-labeled dextran into KB cells. This increased uptake and viral entry depend on passage through an acidic cellular compartment.

    Area of Science:

    • Cell Biology
    • Virology

    Background:

    • Adenovirus interaction with host cells is crucial for viral infection.
    • Understanding early viral entry mechanisms is key to developing antiviral strategies.

    Purpose of the Study:

    • To investigate the early interaction between KB cells and adenovirus.
    • To elucidate the role of acidic compartments in adenovirus entry and macromolecule uptake.

    Main Methods:

    • Utilized KB cells and adenovirus.
    • Measured uptake of fluorescein isothiocyanate-labeled dextran (FD) using fluorescence microscopy.
    • Assessed the effect of pH-altering drugs (chloroquine, ammonium chloride, monensin) on FD uptake and viral entry.

    Main Results:

    • Adenovirus significantly increased cell-associated FD uptake (2-3 fold) compared to controls.
    • Cells incubated with adenovirus showed widespread fluorescence, unlike control cells with localized vesicle fluorescence.
    • Inhibition of acidic compartments with drugs blocked both increased FD uptake and adenovirus nuclear entry.

    Conclusions:

    • Adenovirus entry into KB cells involves passage through an acidic compartment, likely endocytic vesicles.
    • Co-endocytosed macromolecules are released into the cytosol upon viral entry.
    • The findings suggest a pH-dependent mechanism for adenovirus cellular entry.

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