Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Ligand-Gated Ion Channel Receptor: Gating Mechanism01:30

Ligand-Gated Ion Channel Receptor: Gating Mechanism

4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
Ion Channels01:19

Ion Channels

68.1K
The movement of ions like sodium, potassium, and calcium into and out of the cell is essential to maintain the electrochemical gradient in living cells. The ion channels—a class of membrane transport proteins—help maintain this ionic gradient for the smooth functioning of physiological activities such as maintaining cell size and volume, conducting nerve impulses, and gas and nutrient exchange.
Ion channels are specialized integral membrane proteins on the plasma membrane that allow...
68.1K
Patch Clamp01:18

Patch Clamp

6.0K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.0K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

2.8K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Pinocytosis00:38

Pinocytosis

4.0K
Cells use energy-requiring bulk transport mechanisms to transfer large particles or large numbers of small particles into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
Pinocytosis ("cellular drinking") is one of three main types of...
4.0K
Pinocytosis00:43

Pinocytosis

62.8K
Cells use energy-requiring bulk transport mechanisms to transfer large particles, or large amounts of small particles, into or out of the cell. The cells envelop the particles in spherical membranes called vesicles or vacuoles. Vesicles that transport material into the cell are built from the cell membrane. These vesicles encapsulate external molecules and transport them into the cell in a process called endocytosis.
62.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Search for Dark Sector e^{+}e^{-} Explanations of the MiniBooNE Anomaly at MicroBooNE.

Physical review letters·2026
Same author

First Measurement of Charged-Current Muon-Neutrino-Induced K^{+} Production on Argon Using the MicroBooNE Detector.

Physical review letters·2026
Same author

Search for an Anomalous Production of Charged-Current ν_{e} Interactions without Visible Pions across Multiple Kinematic Observables in MicroBooNE.

Physical review letters·2025
Same author

First Measurement of ν_{e} and ν[over ¯]_{e} Charged-Current Single Charged-Pion Production Differential Cross Sections on Argon Using the MicroBooNE Detector.

Physical review letters·2025
Same author

First Double-Differential Cross Section Measurement of Neutral-Current π^{0} Production in Neutrino-Argon Scattering in the MicroBooNE Detector.

Physical review letters·2025
Same author

Measurement of Electron Antineutrino Oscillation Amplitude and Frequency via Neutron Capture on Hydrogen at Daya Bay.

Physical review letters·2024

Related Experiment Video

Updated: May 6, 2026

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

12.6K

Net H(+) influx inNitella clavata.

R K Rent1, R A Johnson, C E Barr

  • 1Department of Biological Sciences, State University College, 14420, Brockport, N.Y..

The Journal of Membrane Biology
|November 2, 2013
PubMed
Summary

Giant algal cells (Nitella clavata) show high membrane permeability to hydrogen ions (H+). Studies indicate minimal internal pH change and significant external H+ adsorption by the membrane.

Area of Science:

  • Plant Physiology
  • Cell Biology
  • Biophysics

Background:

  • The plasma membrane's role in ion transport is crucial for cellular homeostasis.
  • Understanding hydrogen ion (H+) permeability is key to cellular pH regulation.

Purpose of the Study:

  • To quantify net hydrogen ion (H+) influx in Nitella clavata.
  • To investigate the permeability of the Nitella membrane to H+.
  • To explore the membrane's capacity for H+ adsorption.

Main Methods:

  • Net H+ influx measurements using giant internodal cells of Nitella clavata.
  • Exposure of cells to low pH solutions (4.5-4.7).
  • Monitoring pH changes and acid addition required to maintain low pH.

Main Results:

More Related Videos

In Situ Ca2+ Imaging of the Enteric Nervous System
11:26

In Situ Ca2+ Imaging of the Enteric Nervous System

Published on: January 29, 2015

21.5K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.9K

Related Experiment Videos

Last Updated: May 6, 2026

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons
11:35

Flash Photolysis of Caged Compounds in the Cilia of Olfactory Sensory Neurons

Published on: October 29, 2011

12.6K
In Situ Ca2+ Imaging of the Enteric Nervous System
11:26

In Situ Ca2+ Imaging of the Enteric Nervous System

Published on: January 29, 2015

21.5K
Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli
14:05

Morphological and Compositional Analysis of Neutrophil Extracellular Traps Induced by Microbial and Chemical Stimuli

Published on: November 4, 2022

2.9K
  • Measurements align with Kitasato's (1968) findings, supporting high H+ permeability.
  • Internal pH remained largely stable despite external acidic conditions.
  • Evidence suggests significant H+ adsorption capacity on the external membrane surface.

Conclusions:

  • The Nitella membrane exhibits substantial permeability to H+.
  • The cell membrane possesses a notable capacity for adsorbing H+ externally.
  • Internal pH is effectively buffered, indicating robust regulatory mechanisms.