Linkages among RFLP, RAPD, isozyme, disease-resistance, and morphological markers in narrow and wide crosses of...

A 58-point genetic map was constructed with RFLP, RAPD, isozyme, morphological, and disease-resistance markers spanning 766 cM on ten linkage groups for a cross within the cultivated cucumber (Cucumis sativus var. sativus). Relatively few DNA polymorphisms were detected, agreeing with previous studies documenting a narrow genetic base for cucumber. Most RFLPs within the cultivated cucumber appear to be changes at restriction-enzyme sites. Sixty-four percent of RAPD markers that fit expected ratios at P<0.001 were unlinked, possibly due to poor amplification and the inefficiency of dominant markers to detect linkage in an F2 family. A 70-point linkage map, spanning 480 cM on ten linkage groups, was constructed with RFLP, isozyme, morphological, and diseaseresistance markers for a cross between the cultivated cucumber and the wild or feral C. sativus var. hardwickii. Unlinked markers and more linkage groups than chromosome pairs indicated that both maps were not saturated. Twentyone markers doubly segregated in both families and regions of colinearity were identified.