Regeneration of transgenic, microspore-derived, fertile barley
A Jähne1, D Becker, R Brettschneider
1Universität Hamburg, Institut für Allgemeine Botanik, AMP II, Ohnhorststrasse 18, D-22609, Hamburg, Germany.
Abstract:
We have developed a system for the biolistic transformation of barley using freshly-isolated microspores as the target tissue. Independent transformation events led, on average, to the recovery of one plant per 1×10(7) bombarded microspores. Putative transformants have been regenerated using phosphinothricin as a selective agent. R0 plants have been transferred to soil approximately 2 months after bombardment. Integration of the marker genes bar and uidA has been confirmed by Southern analysis. The marker genes are inherited in all progeny plants confirming the expected homozygous nature of the R0 plants.
More Related Videos
10:28Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes
Published on: February 14, 2020
08:52Author Spotlight: Optimizing Hairy Root-Based Transformation Protocols for Enhanced Efficiency in Brassicaceae
Published on: December 22, 2023
Related Concept Videos
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Plant Breeding and Biotechnology
Transgenic Organisms
Bioreactor Controls-III
Gene Regulation During Sporulation
