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Specific and Accurate Detection of the Citrus Greening Pathogen Candidatus liberibacter spp. Using Conventional PCR on Citrus Leaf Tissue Samples

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Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of

Q Cai¹, C L Guy, G A Moore

¹Plant Molecular and Cellular Biology Program, Departments of Environmental and Horticultural Sciences, Institute of Food and Agricultural Sciences, University of Florida, 32611, Gainesville, FL, USA.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 2, 2013

Summary

Researchers developed a genetic linkage map for Citrus using random amplified polymorphic DNA (RAPD) markers. This map aids in identifying genes for cold and salt tolerance in Citrus species.

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Area of Science:

Plant genetics
Molecular biology
Citrus research

Background:

Genetic mapping is crucial for understanding crop traits.
Random amplified polymorphic DNA (RAPD) markers offer a method for genetic analysis.

Purpose of the Study:

To initiate genetic mapping in Citrus using RAPD markers.
To construct a linkage map for identifying genes related to stress tolerance.

Main Methods:

Utilized 140 random primers to generate polymorphic DNA fragments.
Analyzed 60 BC1 progeny from an intergeneric cross for linkage analysis.
Integrated RAPD, RFLP, and isozyme markers to build a core linkage map.

Main Results:

Identified 266 segregating loci from RAPD analysis.
Constructed a core linkage map with nine groups, 109 RAPD, and 51 other markers.
The map spans 1192 cM, covering 70-80% of the genome, with distorted segregation patterns noted.

Conclusions:

A robust genetic linkage map for Citrus has been established.
This map provides a foundation for future quantitative trait loci (QTL) studies for cold and salt tolerance.
Three cold-acclimation-responsive loci were successfully mapped.