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Updated: May 6, 2026

Extraction and Quantification of Soluble, Radiolabeled Inositol Polyphosphates from Different Plant Species using SAX-HPLC
Published on: June 26, 2020
An inositol 1,4,5-trisphosphate-6-kinase activity in pea roots
J A Chattaway1, B K Drøbak, P A Watkins
1Department of Cell Biology, John Innes Institute, Colney Lane, NR4 7UH, Norwich, Norfolk, UK.
Abstract:
A soluble extract from pea (Pisum sativum L.) roots, when incubated with ATP and inositol 1,4,5-trisphosphate, produced an inositol tetrakisphosphate. The chromatographic properties of this inositol tetrakisphosphate, and of the products formed by its chemical degradation, identify it as inositol 1,4,5,6-tetrakisphosphate. No evidence was obtained for a 3-phosphorylation of inositol 1,4,5-trisphosphate. The importance of these observations with respect to inositol phosphates and calcium signalling in higher plants, is discussed.
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