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Updated: May 6, 2026

En Face Detection of Nitric Oxide and Superoxide in Endothelial Layer of Intact Arteries
Published on: February 25, 2016
Correlation between nitric oxide synthase activity and reduced glutathione level in human and murine endothelial
D Ghigo1, D Geromin, C Franchino
1Dipartimento di Genetica, Biologia e Chimica Medica - Sezione di Chimica Medica, Via Santena 5/bis, I-10126, Torino, Italy.
Abstract:
The synthesis of nitric oxide (NO), detected as citrulline production, in human (HUVEC) and murine (tEnd.1) endothelial cells correlated with intracellular GSH. tEnd.1, which exhibited an intracellular GSH level 2.5-fold higher than HUVEC, showed a citrulline production (basally and after ionomycin stimulation) 5-8 times higher than human cells. Ionomycinelicited citrulline synthesis in tEnd.1 cells increased 2.4-fold after loading with GSH, and decreased dose-dependently after GSH depletion. Cell loading with N-(2-mercaptopropionyl)-glycine neither significantly increased citrulline production nor relieved the effect of GSH depletion.

