Related Experiment Video
Updated: May 6, 2026

07:10
Extended Live Imaging of Female Drosophila melanogaster Germline Stem Cell Niches
Published on: December 20, 2024
1.2K
Drosophila germline stem cells for in vitro analyses of PIWI-mediated RNAi
Yuzo Niki1, Takuya Sato, Takafumi Yamaguchi
1Department of Biology, Faculty of Science, Ibaraki University, Mito, Ibaraki, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|November 2, 2013
Abstract:
The Drosophila piwi gene has multiple functions in soma and germ cells. An in vitro system provides a powerful tool for elucidating PIWI function in each cell type using stable cell lines originating from germline stem cells (GSCs) and ovarian soma of adult ovaries. We have described methods for the maintenance and expansion of GSCs in an established cell line (fGS/OSS) and an in situ hybridization method for analyzing piwi.

