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Biochemical and mass spectrometric analysis of 3'-end methylation of piRNAs
Takeo Suzuki1, Kenjyo Miyauchi, Yuriko Sakaguchi
1Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|November 2, 2013
Abstract:
Piwi-interacting RNAs (piRNA) are fully modified by 2'-O-methylation at their 3'-termini. This terminal methylation is required to prevent 3'-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2'-O-methylation at 3'-termini of piRNAs.
