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Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

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Related Experiment Video

Updated: May 6, 2026

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

DNA methylation in mouse testes.

Satomi Kuramochi-Miyagawa¹, Kanako Kita-Kojima, Yusuke Shiromoto

¹Department of Pathology, Medical School, Osaka University, Osaka, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|November 2, 2013

Summary

This summary is machine-generated.

DNA methylation in mouse testes is crucial for regulating retrotransposons and imprinted genes during spermatogenesis. This study details methods to analyze DNA methylation changes in LINE1, IAP, and Rasgrf1.

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Last Updated: May 6, 2026

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Immunohistochemical Detection of 5-Methylcytosine and 5-Hydroxymethylcytosine in Developing and Postmitotic Mouse Retina

Published on: August 29, 2018

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Published on: September 7, 2017

Slide Preparation Method to Preserve Three-dimensional Chromatin Architecture of Testicular Germ Cells

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Published on: January 10, 2014

Area of Science:

Epigenetics
Reproductive Biology
Genomics

Background:

Spermatogenesis involves precise DNA methylation regulation of retrotransposons like LINE1 and IAP, and imprinted genes.
Acquisition of CpG methylation in retrotransposon regulatory regions occurs during embryonic testis development.
Deficiencies in piRNA biogenesis are linked to reduced DNA methylation in LINE1, IAP, and the imprinted gene Rasgrf1.

Purpose of the Study:

To describe robust methods for analyzing DNA methylation in mouse testes.
To investigate DNA methylation patterns of key retrotransposons and imprinted genes.

Main Methods:

Bisulfite sequencing for precise DNA methylation analysis.
Southern blotting utilizing methylation-sensitive restriction enzymes.

Main Results:

Established protocols for assessing DNA methylation in LINE1, IAP, and imprinted genes.
Provided tools to study epigenetic regulation during male germ cell development.

Conclusions:

Accurate DNA methylation analysis is vital for understanding spermatogenesis.
These methods facilitate research into epigenetic defects affecting male fertility.