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Updated: May 6, 2026

Enhanced Crosslinking Immunoprecipitation eCLIP Method for Efficient Identification of Protein-bound RNA in Mouse Testis
Published on: May 10, 2019
Analysis of small RNA-guided endonuclease activity in endogenous Piwi protein complexes from mouse testes
Michael Reuter1, Ramesh S Pillai
1EMBL Grenoble Outstation, Grenoble, Cedex, France.
Abstract:
Small RNAs associate with members of the Argonaute family to function in gene regulation, transposon control, and creation of silent chromatin domains. In this partnership, small RNAs act as guides for the bound Argonaute and other associated proteins. Complementary base pairing of small RNAs to target nucleic acid molecules allow specificity for the small RNA-mediated functions. One key activity of some Argonaute protein family members is their small RNA-guided endonuclease activity called Slicer action. Here we describe a protocol that can be used to probe slicer activity in endogenous Piwi complexes isolated from mouse testes.
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