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UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for...

Fast Reactions

Fast Reactions

Fast reactions occurring in times shorter than the time needed to mix reactants pose a unique challenge for investigation. In a liquid-phase continuous-flow system, reactants A and B are swiftly pushed into the mixing chamber, where mixing occurs within 1 ms. The reaction mixture then flows through an observation tube, and one measures light absorption to determine species concentrations at various points of the tube. This method is most appropriate when relatively large volumes of reactants...

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Related Experiment Video

Updated: May 6, 2026

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Real-time Monitoring of Reactions Performed Using Continuous-flow Processing: The Preparation of 3-Acetylcoumarin as an Example

Published on: November 18, 2015

Microreactors with integrated UV/Vis spectroscopic detection for online process analysis under segmented flow.

Jun Yue¹, Floris H Falke, Jaap C Schouten

¹Laboratory of Chemical Reactor Engineering, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, P.O. Box 513, 5600 MB Eindhoven, The Netherlands. T.A.Nijhuis@tue.nl.

|November 2, 2013

Summary

This summary is machine-generated.

This study integrates UV/Vis spectroscopy with microreactors for real-time analysis in multiphase flow. The developed systems enable efficient process monitoring and characterization, particularly for gold nanoparticle synthesis.

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Area of Science:

Analytical Chemistry
Chemical Engineering
Microfluidics

Background:

Online process analysis is crucial for accelerating microreactor development.
Integrating reaction and detection in multiphase microfluidic flow presents challenges.

Purpose of the Study:

To couple UV/Vis spectroscopy with microreactors for online process analysis under segmented flow.
To demonstrate the capabilities of integrated systems for real-time measurements and flow characterization.

Main Methods:

Two integration schemes were developed: transmission mode using a flow-through cell and evanescent wave detection using embedded waveguides.
Model experiments were conducted to validate the systems.
Gold nanoparticle synthesis was used as an application case.

Main Results:

The integrated systems achieved real-time concentration measurements.
Segmented flow characterization was successfully performed.
The systems demonstrated potential for process monitoring in microreactors.

Conclusions:

UV/Vis spectroscopy can be effectively coupled with microreactors for online analysis in segmented flow.
These integrated systems offer significant potential for process development and monitoring in microfluidic applications.