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Related Concept Videos

Proteomics01:33

Proteomics

7.5K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.5K
Protein-protein Interfaces02:04

Protein-protein Interfaces

12.6K
Many proteins form complexes to carry out their functions, making protein-protein interactions (PPIs) essential for an organism's survival. Most PPIs are stabilized by numerous weak noncovalent chemical forces. The physical shape of the interfaces determines the way two proteins interact. Many globular proteins have closely-matching shapes on their surfaces, which form a large number of weak bonds. Additionally, many PPIs occur between two helices or between a surface cleft and a...
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Related Experiment Video

Updated: May 6, 2026

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
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Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

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Development of proteome-wide binding reagents for research and diagnostics.

Michael J Taussig1, Ronny Schmidt, Elizabeth A Cook

  • 1Protein Technology Group, The Babraham Institute, Cambridge, UK; Cambridge Protein Arrays Ltd, Babraham Research Campus, Cambridge, UK.

Proteomics. Clinical Applications
|November 2, 2013
PubMed
Summary

Developing comprehensive protein binders is crucial for proteomics research. These affinity reagents are essential for understanding protein function, location, and quantity across the entire human proteome.

Keywords:
Affinity reagentAntibodyProteome-wide projectsRecombinant binder

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Area of Science:

  • Proteomics
  • Biotechnology
  • Molecular Biology

Background:

  • Antibodies and protein binders are vital affinity reagents in proteomics for analyzing protein characteristics.
  • Existing research antibodies are often incomplete or of uncertain quality, necessitating new efforts.
  • Advancements in automation enable the potential to create binders for every human protein.

Purpose of the Study:

  • To review the requirements for producing a comprehensive pipeline of protein binders for the human proteome.
  • To discuss ongoing projects and strategies for generating these essential research tools.
  • To highlight the immense benefits of well-characterized, proteome-wide binder resources.

Main Methods:

  • Review of target prioritization strategies.
  • Analysis of antigen design principles.
  • Evaluation of 'next generation' methods and databases.
  • Examination of current European and USA-based projects.

Main Results:

  • The generation of binders for the entire human proteome is a complex but achievable goal.
  • Ongoing projects are making progress in developing these resources.
  • International collaboration is identified as key to success.

Conclusions:

  • Well-characterized, quality-controlled pan-proteome binder resources offer enormous benefits for biomedical research and industry.
  • The development of these resources requires significant technical, personnel, and financial investment.
  • Large-scale international collaboration is recommended to accelerate progress.