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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

PCR - Polymerase Chain Reaction

PCR - Polymerase Chain Reaction

PCR

PCR

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Related Experiment Video

Updated: May 6, 2026

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

Published on: February 23, 2018

Real-time PCR II: probe methods

John Brunstein¹

¹PatholD, Inc, British Columbia.

MLO: Medical Laboratory Observer

|November 5, 2013

Summary

No abstract available in PubMed .

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