Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

4.9K
4.9K
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

14.9K
To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
14.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Frameworks to identify research gaps, frame research needs, and derive research priorities from evidence syntheses: a scoping review.

Journal of clinical epidemiology·2026
Same author

Assessing the trustworthiness of health guidelines recommendations: the transparent, rigorous, useable, standardized, and trustworthy guide (TRUSTGUIDES) tools development.

Journal of clinical epidemiology·2026
Same author

Comparative effectiveness of physical therapy interventions in adults with rotator cuff tendinopathy: a systematic review and network meta-analysis.

British journal of sports medicine·2026
Same author

Delivery arrangements for rehabilitation services in health systems: an overview of systematic reviews.

The Cochrane database of systematic reviews·2026
Same author

Development of a 3D bioprinted human skin model for predictive toxicology.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Sustainable chemistry and preclinical characterization of RC-106·HCl: A brain-penetrant pan sigma receptor modulator for glioblastoma.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences·2026

Related Experiment Video

Updated: May 6, 2026

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments
06:08

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Published on: May 26, 2019

7.0K

In vitro irradiation system for radiobiological experiments.

Anna Tesei1, Anna Sarnelli, Chiara Arienti

  • 1Biosciences Laboratory, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, Biosciences Laboratory, via P, Maroncelli 40, 47014, Meldola, FC, Italy. anna.tesei@irst.emr.it.

Radiation Oncology (London, England)
|November 5, 2013
PubMed
Summary

A novel system irradiates three-dimensional (3-D) tumor spheroids, bridging in vitro and in vivo models for radiobiology. This method enhances understanding of cancer treatment, potentially personalizing radiotherapy by mimicking clinical conditions.

More Related Videos

Dosimetry for Cell Irradiation using Orthovoltage 40-300 kV X-Ray Facilities
06:51

Dosimetry for Cell Irradiation using Orthovoltage 40-300 kV X-Ray Facilities

Published on: February 20, 2021

4.6K
Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition
06:20

Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition

Published on: March 11, 2021

7.4K

Related Experiment Videos

Last Updated: May 6, 2026

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments
06:08

Use of a Linear Accelerator for Conducting In Vitro Radiobiology Experiments

Published on: May 26, 2019

7.0K
Dosimetry for Cell Irradiation using Orthovoltage 40-300 kV X-Ray Facilities
06:51

Dosimetry for Cell Irradiation using Orthovoltage 40-300 kV X-Ray Facilities

Published on: February 20, 2021

4.6K
Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition
06:20

Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition

Published on: March 11, 2021

7.4K

Area of Science:

  • Oncology
  • Radiation Oncology
  • Biomedical Engineering

Background:

  • Two-dimensional (2-D) cell cultures lack the complexity of three-dimensional (3-D) solid tumors, limiting their translational value in radiobiology.
  • There is a critical need for advanced in vitro models that closely replicate clinical conditions for accurate radiobiological studies.

Purpose of the Study:

  • To develop and validate a novel system for sterile irradiation of 3-D tumor spheroids.
  • To bridge the gap between simple 2-D cell cultures and complex in vivo tumors for radiobiological research.

Main Methods:

  • A new system was designed for irradiating 3-D tumor spheroids under sterile conditions, utilizing standard patient treatment equipment.
  • Multicellular tumor spheroids (MCTS) in 96-multiwell plates were irradiated within a custom-built phantom simulating human tissue composition.

Main Results:

  • Irradiation of CAEP spheroids pre-treated with cisplatin showed significantly increased radiotoxicity compared to single treatments.
  • The radio-chemotherapy combination in A549 spheroids yielded results similar to monotherapy.
  • A 20 Gy dose did not impact monolayer cell survival, while cisplatin-based treatments resulted in 100% cell death in both 2-D and 3-D models.

Conclusions:

  • A system for sterile irradiation of 3-D tumor cultures was established, enabling the use of clinical dose intensities and schedules.
  • This irradiation system, combined with 3-D cell cultures, offers potential for generating data to personalize radiotherapy treatments.