Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

7.8K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
7.8K
Real Time RT-PCR02:57

Real Time RT-PCR

52.0K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
52.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Prenatal diagnosis and genetics analysis of a fetus with type 27 intellectual disability syndrome due to variant of SRRM2 gene].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2026
Same author

Temperature-dependent γ' kinetic evolution and elemental partitioning in a novel CoNi-based superalloy: an integrated CALPHAD, phase-field, and experimental study.

Materials horizons·2026
Same author

Electrophysiological Effects of Right and Left Tragus Nerve Stimulation in Humans.

Circulation. Arrhythmia and electrophysiology·2026
Same author

A High-Sensitivity MXene/PVDF Flexible Piezoelectric Sensor for Intelligent Tunnel Lighting.

Materials (Basel, Switzerland)·2026
Same author

Application of a modified MSRE-qPCR method for detecting circulating cell-free DNA methylation in cervical cancer.

Frontiers in oncology·2026
Same author

Bio-derived tannic acid-Eu(iii) coordination synergizes with aluminum diethylphosphinate for low-loading and high-efficiency flame retardancy in PA66.

RSC advances·2026

Related Experiment Video

Updated: May 6, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.2K

Multiplexed target detection using DNA-binding dye chemistry in droplet digital PCR.

Geoffrey P McDermott1, Duc Do, Claudia M Litterst

  • 1The Digital Biology Center, Bio-Rad Laboratories, Inc. , 5731 West Las Positas Boulevard, Pleasanton, California 94566, United States.

Analytical Chemistry
|November 5, 2013
PubMed
Summary

The new droplet digital PCR (ddPCR) system now supports DNA-binding dye detection, enabling absolute quantification of multiple nucleic acid targets in a single reaction. This advancement improves precision and accounts for nonspecific amplification, enhancing genetic research capabilities.

More Related Videos

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

9.9K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

6.6K

Related Experiment Videos

Last Updated: May 6, 2026

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions
08:23

Single Droplet Digital Polymerase Chain Reaction for Comprehensive and Simultaneous Detection of Mutations in Hotspot Regions

Published on: September 25, 2018

13.2K
Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

9.9K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

6.6K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Quantitative real-time PCR (qPCR) presents uncertainties in nucleic acid quantification.
  • The first droplet digital PCR (ddPCR) system offered absolute quantification using TaqMan probes.
  • Advancements in droplet chemistry and dye-based detection have driven innovation.

Purpose of the Study:

  • To introduce a second-generation ddPCR system compatible with both TaqMan probes and DNA-binding dyes.
  • To characterize the performance of DNA-binding dye-based ddPCR.
  • To compare DNA-binding dye ddPCR with TaqMan probe-based ddPCR.

Main Methods:

  • Development of a "second generation" ddPCR system.
  • Utilizing DNA-binding dye chemistry for target detection.
  • Partitioning samples prior to thermal cycling for multiplexing.
  • Side-by-side comparison with TaqMan probe detection methods.

Main Results:

  • Demonstrated successful quantification of multiple targets using DNA-binding dye in a single reaction.
  • Showcased the ability to visualize and correct for nonspecific amplification products due to increased resolution.
  • Provided a comparative analysis of DNA-binding dye versus TaqMan probe detection in ddPCR.

Conclusions:

  • The enhanced ddPCR system integrates DNA-binding dye chemistry, expanding its utility.
  • This advancement allows for precise, absolute quantification of multiple nucleic acid targets simultaneously.
  • The improved ddPCR system is poised to facilitate complex genetic research by combining precision with versatile detection chemistries.