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Updated: May 6, 2026
Quantifying the Antifungal Activity of Peptides Against Candida albicans
Published on: January 13, 2023
Antifungal activity of several root canal sealers against Candida albicans
Erhan Ozcan1, Erkan Yula, Zeki Arslanoğlu
1Department of Endodontics, Faculty of Dentistry, Selçuk University , Konya , Turkey.
Objective:
The purpose of this in vitro study was to evaluate the antifungal activity of several root canal sealers (iRoot SP, MTA Fillapex and GuttaFlow) against Candida albicans and compare them to that of AH Plus Jet.
Materials And Methods:
A 10 μL fungi in suspension was allowed to directly contact the sealers, which were freshly mixed or allowed to set for 1 or 7 days. Fresh media was then added and survival of fungi was determined by using 10-fold serial dilution and inoculated onto agar plates. After incubation for 48 h, colony-forming units (CFU) were calculated and their log10 values converted. The data were analyzed using ANOVA and Tukey tests (α = 0.05).
Results:
Freshly mixed AH Plus Jet totally inhibited the growth of fungi and showed the highest antifungal activity. GuttaFlow did not show any significant antifungal activity at all times. Freshly mixed iRoot SP and MTA Fillapex were found to be antifungal. Statistical differences were found between freshly mixed and set samples (p < 0.05) in favor of the former, except GuttaFlow. No statistically significant differences were found among the tested sealers at the 1 and 7-day samples (p > 0.05).
Conclusions:
Fresh AH Plus Jet had very potent antifungal activity. All sealers, except GuttaFlow, exhibited antifungal activity when freshly mixed.
Insights
Freshly mixed AH Plus Jet demonstrated potent antifungal activity against Candida albicans. Other root canal sealers like iRoot SP and MTA Fillapex also showed antifungal properties when initially mixed.
Area of Science:
- Endodontics
- Microbiology
- Dental Materials Science
Background:
- Root canal sealers are crucial for the success of endodontic treatment.
- Candida albicans is a common opportunistic fungal pathogen in persistent endodontic infections.
- Evaluating the antifungal properties of root canal sealers is essential for preventing and treating endodontic complications.
Purpose of the Study:
- To assess the in vitro antifungal efficacy of iRoot SP, MTA Fillapex, and GuttaFlow root canal sealers against Candida albicans.
- To compare the antifungal activity of these sealers with AH Plus Jet.
- To determine the influence of sealer setting time on antifungal properties.
Main Methods:
- An in vitro study design was employed.
- Freshly mixed and set (1 and 7 days) root canal sealers were exposed to Candida albicans.
- Fungal survival was quantified using serial dilution and colony-forming unit (CFU) counts.
Main Results:
- Freshly mixed AH Plus Jet exhibited the highest antifungal activity, completely inhibiting fungal growth.
- iRoot SP and MTA Fillapex showed antifungal activity when freshly mixed.
- GuttaFlow displayed no significant antifungal activity at any time point.
- Antifungal activity was significantly higher for freshly mixed sealers compared to set sealers (p < 0.05), except for GuttaFlow.
Conclusions:
- Fresh AH Plus Jet possesses potent antifungal properties against Candida albicans.
- iRoot SP and MTA Fillapex demonstrate antifungal activity in their freshly mixed state.
- GuttaFlow showed no significant antifungal effect.
- The antifungal efficacy of most sealers is diminished after setting.
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