Related Experiment Video
Updated: May 6, 2026

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
Published on: October 5, 2011
Somatic cell nuclear transfer-derived embryonic stem cell lines in humans: pros and cons
Alena Langerova1, Helena Fulka, Josef Fulka
11 GENNET , 170 00 Prague 7, Czech Republic .
Abstract:
The recent paper, published by Mitalipov's group in Cell (Tachibana et al., 2013 ), reporting the production of human somatic cell nuclear transfer (SCNT) embryonic stem cells (ESCs), opens again the debate if, in the era of induced pluripotent stem cells (iPSCs), the production of these cells is indeed necessary and, if so, whether they are different from ESCs produced from spare embryos and iPSCs. It is our opinion that these questions are very difficult to answer because it is still unclear whether and how normal ESCs differ from iPSCs.
More Related Videos
08:00Scalable 96-well Plate Based iPSC Culture and Production Using a Robotic Liquid Handling System
Published on: May 14, 2015
10:48Derivation and Characterization of a Transgene-free Human Induced Pluripotent Stem Cell Line and Conversion into Defined Clinical-grade Conditions
Published on: November 26, 2014
Related Concept Videos
Embryonic Stem Cells
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
Embryonic Stem Cells
Induced Pluripotent Stem Cells
Somatic...
Methods of Nuclear Reprogramming
Reproductive Cloning
Somatic Cell Nuclear Transfer
In SCNT, an egg cell is taken from an animal and its nucleus is removed, creating an enucleated egg. Then a somatic...
Introduction to Nuclear Reprogramming