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Storage of bacteria and yeast
1Department of Pathology, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods in Enzymology
|November 5, 2013
Summary
Yeast and bacteria can be preserved long-term using cryopreservation. Preparing stocks is essential for archival purposes, ensuring indefinite storage of microbial cultures.
Area of Science:
- Microbiology
- Cryobiology
Background:
- Microbial cultures, including yeast and bacteria, are vital for research and industry.
- Long-term preservation methods are needed to maintain the viability of these cultures.
Purpose of the Study:
- To highlight the utility of cryopreservation for long-term microbial stock storage.
- To emphasize the importance of preparing archival stocks for yeast and bacteria.
Main Methods:
- Cryopreservation techniques applied to yeast and bacterial cultures.
- Storage protocols for maintaining viability over extended periods.
Main Results:
- Demonstrated indefinite storage potential for cryopreserved yeast and bacteria.
- Established the feasibility of creating stable microbial archives.
Conclusions:
- Cryopreservation offers a reliable method for the indefinite storage of yeast and bacterial stocks.
- Archival stock preparation is crucial for preserving microbial genetic resources.
Keywords:
Bacterial and yeast cellsCryopreservation of bacterial culturesCryopreservation of yeastGlycerolGrowth of bacterial and yeast culturesStorage of bacteriaYeastMore Related Videos
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