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Preparation of slides for microscopy from frozen tissue sections
1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Methods in Enzymology
|November 5, 2013
Abstract:
Freezing tissue is a convenient method for long-term storage. In addition, sectioning tissue under frozen conditions or embedded within a frozen matrix allows for stabilization of tissue during the sectioning process. This is advantageous for small and/or delicate tissue (e.g., rodent brains younger than postnatal day 7) and tissue that will be sectioned very thin (usually <150 μm).
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