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Preparation of formalin-fixed paraffin-embedded tissue for immunohistochemistry

Kirstie Canene-Adams1

  • 1Department of Pathology, Johns Hopkins School of Medicine, Baltimore, MD, USA.

Methods in Enzymology
|November 5, 2013
PubMed

Keywords:
Fixation of tissueFormalin-fixed paraffin-embedded tissue (FFPET)ImmunohistochemistryTissue process and embeddedTissue section

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The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
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