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Vesicle extrusion through polycarbonate track-etched membranes using a hand-held mini-extruder
Ting F Zhu1, Itay Budin, Jack W Szostak
1Department of Molecular Biology, Howard Hughes Medical Institute, Massachusetts General Hospital, Boston, MA, USA.
Methods in Enzymology
|November 5, 2013
Abstract
None:
The vesicle extrusion method is used for preparing small (e.g., 100 nm diameter) monodisperse (uniform-sized) vesicles. Polycarbonate track-etched membranes with pores generally ranging from 50 to 200 nm in diameter and a hand-held mini-extruder are used for such a procedure. Extrusion through small (100 nm in diameter or smaller) pores also ensures that the vesicle population is predominantly unilamellar.
