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Androgen-repressed messages in the rat ventral prostate.
The Prostate
|January 1, 1986
Summary
Castration causes prostate steroid-binding protein (PSBP) and other androgen-dependent proteins to disappear in rats. New androgen-repressed RNA sequences emerge, potentially contributing to hormone-resistant prostate cancer.
Area of Science:
- Molecular Biology
- Endocrinology
- Proteomics
Background:
- Prostate function is regulated by androgens.
- Androgen withdrawal affects gene expression in the prostate.
Purpose of the Study:
- To investigate changes in prostate gene expression following castration.
- To identify specific RNA sequences and proteins affected by androgen levels.
Main Methods:
- Translation of polyadenylated RNA (poly(A)+ RNA) from rat prostates.
- Electrophoresis and fluorography of translation products.
- Criss-cross liquid hybridization and Northern hybridization analyses.
Main Results:
- Castration led to the disappearance of prostate steroid-binding proteins and other androgen-dependent proteins.
- Two major (Mr 40,000 and 45,000) and several minor proteins appeared in castrated rat prostate RNA translation products.
- Androgen-repressed poly(A)+ RNA sequences, including a prominent 2,000-nucleotide RNA, were identified and quantified post-castration.
Conclusions:
- Androgen withdrawal significantly alters prostate gene expression at both the RNA and protein levels.
- The identified androgen-repressed RNA sequences may play a role in the development of hormone resistance in human prostate cancer.