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Updated: May 6, 2026
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
Published on: April 17, 2009
Genotypic and media factors affecting stabilization of polyembryogenic cultures in norway spruce (Picea abies (L.)
1Department of Reforestation, Institute of Forestry, Institutski Ave. 21, 194021, St. Petersburg, Russia.
Abstract:
Embryonal-suspensor mass lines (ESM lines) from mature embryos of three open-pollinated families of Norway spruce [Picea abies (L.) Karst.] were stabilized on several culture media varying in nutrient composition. A pattern of relationship between parameters of polyembryogenic culture performance and medium composition was genetically determined. Stabilized ESM lines could be classified into two phenovariants: (i) lines of homogenous ESM composed entirely of somatic proembryos, and (ii) lines of heterogeneous ESM composed of a mixture of nonembryogenic callus and aggregates of somatic proembryos. The formation of one or the other phenovariant was independent of genotype, but strongly determined by the medium composition.
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