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Updated: May 6, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

RNA-mediated gene activation.

Alan L Jiao¹, Frank J Slack¹

¹Department of Molecular, Cellular and Developmental Biology; Yale University; New Haven, CT USA.

|November 5, 2013

Summary

This summary is machine-generated.

Non-coding RNAs (ncRNAs) are key regulators of gene expression. This review explores how small RNAs, long non-coding RNAs, and enhancer RNAs activate gene expression, focusing on epigenetic mechanisms.

Keywords:

RNAa eRNA enhancer epigenetic lincRNA non-coding RNA saRNA transcriptional activation

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Area of Science:

Molecular Biology
Epigenetics
Genomics

Background:

Non-coding RNAs (ncRNAs) are increasingly recognized as crucial regulators of gene expression.
While RNA-mediated gene silencing is well-understood, ncRNA-mediated gene activation mechanisms remain largely unexplored.
This gap highlights a need for comprehensive review of ncRNA roles in gene activation.

Purpose of the Study:

To summarize current knowledge on gene activation mediated by various types of ncRNAs.
To emphasize the epigenetic mechanisms involved in ncRNA-driven gene activation.
To provide a consolidated overview for researchers in the field.

Main Methods:

Literature review and synthesis of existing research.
Focus on studies investigating small RNAs, long non-coding RNAs, and enhancer-derived RNAs.
Analysis of epigenetic modifications and regulatory pathways.

Main Results:

ncRNAs, including small RNAs, long non-coding RNAs, and enhancer-derived RNAs, can actively promote gene expression.
Epigenetic mechanisms, such as DNA methylation and histone modification, are central to ncRNA-mediated gene activation.
Diverse pathways illustrate the multifaceted roles of ncRNAs beyond silencing.

Conclusions:

ncRNAs play significant, yet underappreciated, roles in activating gene expression through epigenetic modulation.
Further research into ncRNA-mediated activation is essential for a complete understanding of gene regulation.
This review consolidates current findings, paving the way for future investigations.