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Updated: May 6, 2026

Establishment of a Clonal Culture of Unicellular Conjugating Algae
Published on: July 14, 2018
Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales)
T Fujimura1, T Kawai, T Kajiwara
1Laboratory of Marine Biotechnology, Shiono Koryo Kaisha, Ltd., Niitaka, Yodogawa-ku, 532, Osaka, Japan.
Abstract:
Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 10(7) cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
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