Somatic embryogenesis ofCyclamen persicum Mill. 'Anneke' from aseptic seedlings
T Takamura1, I Miyajima, E Matsuo
1Laboratory of Horticultural Science, Faculty of Agriculture, Kyushu University, 812, Hakozaki, Fukuoka, Japan.
Abstract:
InCyclamen persicum 'Anneke', explants from the various vegetative organs of aseptic seedling formed embryoids. The optimal responses were recorded in Murashige and Skoog (MS) medium enriched with 5.0µM 2,4-dichlorophenoxyacetic acid (2,4-D), 0.5µM kinetin and 3-6% sucrose. Embryogenesis was enhanced at higher temperature of 25-30°C. On the other hand, light inhibited embryogenesis. Histological and morphological studies confirmed that the embryoids were indeed somatic embryos.
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