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Thidiazuron-induced high frequency of shoot induction and plant regeneration in protoplast derived pea callus
P Böhmer1, B Meyer, H J Jacobsen
1Lehrgebiet Molekulargenetik, Universität Hannover, Herrenhäuserstr. 2, D-30419, Hannover, Germany.
Abstract:
Protoplasts isolated from lateral shoot buds of cotyledon-free pea embryo axes were regenerated to callus. Protoplast derived calluses with a diameter of about 1cm were transferred to shoot induction media, containing different concentrations (1-50µM) of thidiazuron. Shoot formation was observed after 16 weeks up to 12% efficiency. Thidiazuron (10µM) was the most effective concentration in all experiments. Shoot buds elongated in medium supplemented with N-isopentenyl adenine and indole-3-butyric acid. Since rooting was almost impossible in these thidiazuron-induced shoots, shoots were grafted onto young pea seedlings and regenerated to fertile plants.
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