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Genetic transformation ofPelargonium X hortorum
M P Robichon1, J P Renou, R Jalouzot
1INRA, C.R. d'Angers, Station d'Amélioration des Espèces Fruitières et Ornementales, F-49071, Beaucouzé, France.
Plant Cell Reports
|November 5, 2013
Summary
Transgenic Pelargonium X hortorum plants were successfully produced using Agrobacterium tumefaciens-mediated transformation. This method achieved high regeneration rates and resulted in fertile, phenotypically normal transgenic plants.
Area of Science:
- Plant biotechnology
- Genetic engineering
- Horticultural science
Background:
- Pelargonium X hortorum (garden geranium) is a popular ornamental plant.
- Efficient methods for genetic transformation are crucial for crop improvement and research.
Purpose of the Study:
- To develop and optimize an Agrobacterium tumefaciens-mediated transformation protocol for Pelargonium X hortorum.
- To assess the efficiency of regeneration and genetic integration in transgenic Pelargonium plants.
Main Methods:
- Agrobacterium tumefaciens-mediated transformation of Pelargonium X hortorum using cotyledon and hypocotyl explants.
- Regeneration protocol with a high success rate (approximately 95%).
- Selection of putative transformants using hygromycin and confirmation of foreign DNA insertion via Southern and PCR analysis.
Main Results:
- Transgenic Pelargonium plants were produced with up to 20% efficiency from inoculated explants.
- Southern and PCR analyses confirmed T-DNA insertion, though often not full-length.
- All regenerated (R0) transgenic plants displayed normal phenotypes and were fertile.
Conclusions:
- Agrobacterium tumefaciens-mediated transformation is an effective method for generating transgenic Pelargonium X hortorum.
- The established protocol yields fertile transgenic plants with potential for further research and breeding.
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