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Updated: May 6, 2026

Scalable Transfection of Maize Mesophyll Protoplasts
Published on: June 23, 2023
Transgenic barley (Hordeum vulgare L.) by electroporation of protoplasts
M Salmenkallio-Marttila1, K Aspegren, S Akerman
1VTT Biotechnology and Food Research, P.O. Box 1505, FIN-02044, VTT, Espoo, Finland.
Abstract:
Protoplasts isolated from calli derived from cultured microspores of barley (Hordeum vulgare L. cv. Kymppi, an elite cultivar) were transformed with the neomycin phosphotransferase marker gene (nptII) by electroporation. Screening of the regenerated plants for the NPTII activity by gel assay resulted in three positive signals. Southern blot analysis and NPTII assays of second and third generation plants confirmed the genomic integration of the transferred gene and that the new trait was inherited by the progeny.
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