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Updated: May 6, 2026

Comparative RNA Structure Analysis of Nascent and Mature Transcripts in Saccharomyces cerevisiae
Published on: February 27, 2026
Characterisation of the LdsRNA encoded mRNA of yeast
1Department of Plant Biology and Microbiology, Queen Mary College, University of London, Mile End Road, London El 4NS, Great Britain.
Abstract:
Radioactive messenger RNA (mRNA) was isolated from a Saccharomyces cerevisiae strain containing the L double stranded RNA (dsRNA) species. This mRNA was hybridised to LdsRNA isolated from the same strain. Analysis of the hybrid formed shows it to be of a similar size to the LdsRNA. It is concluded from this result that the mRNA complementary to the -ve strand of the LdsRNA is polycistronic.When the total mRNA is fractionated on oligo (dT) cellulose, RNA complementary to the -ve strand of LdsRNA is found only in the non-binding fraction. When LdsRNA is fractionated on oligo (dT) cellulose the majority is found in the non-binding fraction. It is concluded that neither the LdsRNA genome nor the mRNA it encodes have poly(A) tails of a significant length.
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