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Some of Mendel’s crosses examined three pairs of contrasting characteristics. Such a cross is called a trihybrid cross. A trihybrid cross is a combination of three individual monohybrid crosses. For example, plant height (tall vs. short), seed shape (round vs. wrinkled), and seed color (yellow vs. green).
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Updated: May 6, 2026

Processing the Loblolly Pine PtGen2 cDNA Microarray

Processing the Loblolly Pine PtGen2 cDNA Microarray

Published on: March 20, 2009

An RFLP linkage map for loblolly pine based on a three-generation outbred pedigree.

M E Devey¹, T A Fiddler, B H Liu

¹Division of Forestry/CSIRO, Queen Victoria Terrace, P.O. Box 4008, 2600, Canberra, ACT, Australia.

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik

|November 5, 2013

Summary

This summary is machine-generated.

Researchers created a genetic linkage map for loblolly pine (Pinus taeda L.) using restriction fragment length polymorphism (RFLP) markers. This map provides valuable codominant markers for future genetic studies in this important timber species.

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Area of Science:

Plant Genetics
Forestry
Molecular Biology

Background:

Loblolly pine (Pinus taeda L.) is a vital commercial timber species.
Developing genetic maps is crucial for understanding its complex genome and improving breeding strategies.

Purpose of the Study:

To construct a high-density genetic linkage map for loblolly pine.
To identify and map restriction fragment length polymorphism (RFLP) and isozyme loci.
To provide a set of codominant markers for future genetic analyses.

Main Methods:

Utilized segregation data from a three-generation outbred pedigree (4 grandparents, 2 parents, 95 F2 progeny).
Employed restriction fragment length polymorphism (RFLP) analysis using cDNA and genomic DNA probes.
Scored six polymorphic isozyme loci.
Performed multipoint linkage analysis to establish linkage groups.

Main Results:

A genetic linkage map was constructed with 73 RFLP and 2 isozyme loci placed into 20 linkage groups.
Ninety RFLP loci were detected using 68 probes, with 24% of cDNA probes revealing multiple loci, indicating common multigene families.
High levels of allelic diversity were observed, with up to six alleles per locus in grandparents and three to four in progeny.

Conclusions:

The developed genetic map represents a significant advancement for loblolly pine genetics.
The identified RFLP probes serve as new codominant markers, facilitating advanced genetic studies and marker-assisted selection.
The findings highlight the prevalence of multigene families and extensive allelic variation in loblolly pine, offering insights for breeding programs.