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Updated: May 6, 2026
Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe
Published on: September 13, 2022
r-DNA plasmid from Schizosaccharomyces pombe: Cloning and use in yeast transformation
P Fournier1, C Gaillardin, L de Louvencourt
1Laboratoire de Génétique, Institut National Agronomique, 16 Rue Claude Bernard, F-75231, Paris Cedex 05, France.
Abstract:
1. an extrachromosomal, extramitochondrial DNA circle of about 3 μm length, occurring in S. pombe, was cloned in pBR322 and a detailed restriction map of the cloned sequence constructed. 2. Hybridization of this DNA with the ribosomal DNA from S. cerevisiae revealed regions of homology which were mapped in the cloned S. pombe DNA. 3. A transformation system was developed using the URA3 gene of S. cerevisiae and the 3 μm molecule. It was shown that the r-DNA part of the hybrid plasmid promotes replication in S. pombe.
